Nuclear-Envelope Localization of an Adenovirus Tumor-Antigen Maintains the Integrity of Cellular DNA

White, E., Blose, S. H., Stillman, B. W. (1984) Nuclear-Envelope Localization of an Adenovirus Tumor-Antigen Maintains the Integrity of Cellular DNA. Molecular and Cellular Biology, 4 (12). pp. 2865-2875. ISSN 0270-7306

URL: http://www.ncbi.nlm.nih.gov/pubmed/6396510
DOI: 10.1128/MCB.4.12.2865

Abstract

The adenovirus early-region 1B 19,000-molecular-weight tumor antigen is required for oncogenic transformation of cells by adenovirus. We have demonstrated that this tumor antigen is located in the nuclear envelope of infected and transformed cells and that a fraction of the protein within the nuclear envelope is associated with the nuclear lamina. During cell division in the transformed cells, the nuclear envelope containing the tumor antigen dissociates at metaphase and then reforms around the separated daughter chromosomes at telophase. Adenovirus mutants carrying lesions in the gene encoding this tumor antigen cause degradation of host cell chromosomal DNA, and in these mutants, the intracellular localization of the 19,000-dalton protein is altered. These results demonstrate that components of the nuclear envelope function in the organization of chromatin in infected and transformed cells and that a virus-encoded protein plays a critical role in this process.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > cDNA
organism description > virus
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 17 Feb 2012 19:21
Last Modified: 20 Jun 2017 20:39
PMCID: PMC369299
Related URLs:
URI: http://repository.cshl.edu/id/eprint/25136

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving