Chromatin assembly coupled to DNA repair: a new role for chromatin assembly factor I

Gaillard, P. H., Martini, E. M., Kaufman, P. D., Stillman, B., Moustacchi, E., Almouzni, G. (1996) Chromatin assembly coupled to DNA repair: a new role for chromatin assembly factor I. Cell, 86 (6). pp. 887-96. ISSN 0092-8674 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8808624
DOI: 10.1016/S0092-8674(00)80164-6

Abstract

DNA repair in the eukaryotic cell disrupts local chromatin organization. To investigate whether the resetting of nucleosomal arrays can be linked to the repair process, we developed model systems, with both Xenopus egg extract and human cell extracts, to follow repair and chromatin assembly in parallel on circular DNA templates. Both systems were able to carry out nucleotide excision repair of DNA lesions. We observed that UV-dependent DNA synthesis occurs simultaneously with chromatin assembly, strongly indicating a mechanistic coupling between the two processes. A complementation assay established that chromatin assembly factor I (CAF1) is necessary for this repair associated chromatin formation.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Line Chromatin metabolism Chromosomal Proteins Non-Histone DNA Repair physiology DNA-Binding Proteins metabolism Female Humans Models Biological Oocytes metabolism Plasmids metabolism radiation effects Recombinant Proteins metabolism Ultraviolet Rays Xenopus
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > nucleosome
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date Deposited: 06 Mar 2012 17:04
Last Modified: 20 Jun 2017 19:37
Related URLs:
URI: http://repository.cshl.edu/id/eprint/24980

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