High-throughput fluorescent tagging of full-length arabidopsis gene products in planta

Tian, G. W., Mohanty, A., Chary, S. N., Li, S. J., Paap, B., Drakakaki, G., Kopec, C. D., Li, J. X., Ehrhardt, D., Jackson, D., Rhee, S. Y., Raikhel, N. V., Citovsky, V. (May 2004) High-throughput fluorescent tagging of full-length arabidopsis gene products in planta. Plant Physiology, 135 (1). pp. 25-38. ISSN 0032-0889

URL: http://www.ncbi.nlm.nih.gov/pubmed/15141064
DOI: 10.1104/pp.104.040139

Abstract

We developed a high-throughput methodology, termed fluorescent tagging of full-length proteins (FTFLP), to analyze expression patterns and subcellular localization of Arabidopsis gene products in planta. Determination of these parameters is a logical first step in functional characterization of the approximately one-third of all known Arabidopsis genes that encode novel proteins of unknown function. Our FTFLP-based approach offers two significant advantages: first, it produces internally-tagged full-length proteins that are likely to exhibit native intracellular localization, and second, it yields information about the tissue specificity of gene expression by the use of native promoters. To demonstrate how FTFLP may be used for characterization of the Arabidopsis proteome, we tagged a series of known proteins with diverse subcellular targeting patterns as well as several proteins with unknown function and unassigned subcellular localization.

Item Type: Paper
Uncontrolled Keywords: SUBCELLULAR-LOCALIZATION subcellular localization PROTEIN LOCALIZATION protein localization GLUTAMINE-SYNTHETASE glutamine synthase MOVEMENT PROTEINS movement proteins NUCLEAR-ENVELOPE nuclear envelope BINDING-PROTEIN binding protein BUDDING YEAST budding yeast IN-VIVO in-vivo EXPRESSION expression cells CELLS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA expression
bioinformatics > genomics and proteomics > annotation > sequence annotation
bioinformatics > genomics and proteomics > Mapping and Rendering > Sequence Rendering
CSHL Authors:
Communities: CSHL labs > Jackson lab
Watson School > Publications
Depositing User: CSHL Librarian
Date: May 2004
Date Deposited: 25 Jan 2012 19:02
Last Modified: 19 Sep 2014 15:13
PMCID: PMC429330
Related URLs:
URI: http://repository.cshl.edu/id/eprint/22503

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