The temporal structure of the inner retina at a single glance

Zhao, Zhijian, Klindt, David A, Maia Chagas, André, Szatko, Klaudia P, Rogerson, Luke, Protti, Dario A, Behrens, Christian, Dalkara, Deniz, Schubert, Timm, Bethge, Matthias, Franke, Katrin, Berens, Philipp, Ecker, Alexander S, Euler, Thomas (March 2020) The temporal structure of the inner retina at a single glance. Scientific Reports, 10 (1). p. 4399. ISSN 2045-2322

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Abstract

The retina decomposes visual stimuli into parallel channels that encode different features of the visual environment. Central to this computation is the synaptic processing in a dense layer of neuropil, the so-called inner plexiform layer (IPL). Here, different types of bipolar cells stratifying at distinct depths relay the excitatory feedforward drive from photoreceptors to amacrine and ganglion cells. Current experimental techniques for studying processing in the IPL do not allow imaging the entire IPL simultaneously in the intact tissue. Here, we extend a two-photon microscope with an electrically tunable lens allowing us to obtain optical vertical slices of the IPL, which provide a complete picture of the response diversity of bipolar cells at a "single glance". The nature of these axial recordings additionally allowed us to isolate and investigate batch effects, i.e. inter-experimental variations resulting in systematic differences in response speed. As a proof of principle, we developed a simple model that disentangles biological from experimental causes of variability and allowed us to recover the characteristic gradient of response speeds across the IPL with higher precision than before. Our new framework will make it possible to study the computations performed in the central synaptic layer of the retina more efficiently.

Item Type:	Paper
Subjects:	organism description > animal organism description > animal > mammal organism description > animal > mammal > rodent > mouse organs, tissues, organelles, cell types and functions organs, tissues, organelles, cell types and functions > tissues types and functions > retina organism description > animal > mammal > rodent organs, tissues, organelles, cell types and functions > tissues types and functions
CSHL Authors:	Klindt, David
Communities:	CSHL labs > Klindt lab
SWORD Depositor:	CSHL Elements
Depositing User:	CSHL Elements
Date:	10 March 2020
Date Deposited:	11 Apr 2024 15:47
Last Modified:	11 Apr 2024 15:47
PMCID:	PMC7064538
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/41498

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