Preparation and characterization of green fluorescent nanodiamonds for biological applications

Wee, Tse-Luen, Mau, Yi-Wen, Fang, Chia-Yi, Hsu, Hsiang-Ling, Han, Chau-Chung, Chang, Huan-Cheng (2009) Preparation and characterization of green fluorescent nanodiamonds for biological applications. Diamond and Related Materials, 18 (2-3). pp. 567-573. ISSN 0925-9635

DOI: 10.1016/j.diamond.2008.08.012


Green fluorescent nanodiamonds (denoted as gFNDs) have been produced and characterized for use as cellular markers and/or labels. The gFND particles, containing a high concentration of N-V-N (or H3) centers, were prepared by radiation damage of type Ia natural diamond nanocrystallites with either a home-built 40-keV He+ beam or a research-grade 3-MeV H+ beam, followed by thermal annealing at 800 °C. Prior to the irradiation treatment, infrared spectroscopy of C-N stretches (at 1282 cm- 1) of natural diamond single crystals revealed a nitrogen concentration in the range of 900 ppm. Irradiation and annealing of the specimens led to the emergence of a broad absorption band at ~ 470 nm, corresponding to the phonon sideband of the electronic transition of the H3 center. Measuring the integrated absorption coefficient of the zero-phonon line (at 503 nm) of this center at liquid nitrogen temperature suggested a H3 density of 1.7 × 1018 centers/cm3 (or 10 ppm). A similarly high concentration of the defect centers was estimated for natural diamond nanocrystallites treated under the same experimental conditions. The centers emitted green light (λem = 530 nm) upon illumination by a blue laser (λex = 473 nm or 488 nm). Applications of this novel nanomaterial as fluorescent cellular markers were demonstrated with both confocal fluorescence microscopy and flow cytometry of 70-nm-sized gFND particles uptaken by live HeLa cells through endocytosis.

Item Type: Paper
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > microscopy > flourescence microscopy
Investigative techniques and equipment > microscopy
CSHL Authors:
Communities: CSHL labs > Spector lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 2009
Date Deposited: 04 Jan 2024 20:47
Last Modified: 04 Jan 2024 20:47
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