Deagle, Rebecca C, Wee, Tse-Luen Erika, Brown, Claire M (August 2017) Reproducibility in light microscopy: Maintenance, standards and SOPs. International Journal of Biochemistry and Cell Biology, 89. pp. 120-124. ISSN 1357-2725
Abstract
Light microscopy has grown to be a valuable asset in both the physical and life sciences. It is a highly quantitative method available in individual research laboratories and often centralized in core facilities. However, although quantitative microscopy is becoming a customary tool in research, it is rarely standardized. To achieve accurate quantitative microscopy data and reproducible results, three levels of standardization must be considered: (1) aspects of the microscope, (2) the sample, and (3) the detector. The accuracy of the data is only as reliable as the imaging system itself, thereby imposing the need for routine standard performance testing. Depending on the task some maintenance procedures should be performed once a month, some before each imaging session, while others conducted annually. This text should be implemented as a resource for researchers to integrate with their own standard operating procedures to ensure the highest quality quantitative microscopy data.
| Item Type: | Paper |
|---|---|
| Subjects: | Investigative techniques and equipment Investigative techniques and equipment > microscopy |
| CSHL Authors: | |
| Communities: | CSHL labs > Spector lab |
| SWORD Depositor: | CSHL Elements |
| Depositing User: | CSHL Elements |
| Date: | August 2017 |
| Date Deposited: | 04 Jan 2024 20:26 |
| Last Modified: | 04 Jan 2024 20:26 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/41380 |
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