An immunocompetent rectal cancer model to study radiation therapy

Kim, JK, Wu, C, Del Latto, M, Gao, Y, Choi, SH, Kierstead, M, Gabriel Sauvé, CE, Firat, C, Perez, AC, Sillanpaa, J, Chen, CT, Lawrence, KE, Paty, PB, Barriga, FM, Wilkinson, JE, Shia, J, Sawyers, CL, Lowe, SW, García-Aguilar, J, Romesser, PB, Smith, JJ (December 2022) An immunocompetent rectal cancer model to study radiation therapy. Cell Reports: Methods, 2 (12). p. 100353. ISSN 2667-2375

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DOI: 10.1016/j.crmeth.2022.100353


We describe a mouse model of rectal cancer (RC) involving rapid tumor organoid engraftment via orthotopic transplantation in an immunocompetent setting. This approach uses simple mechanical disruption to allow engraftment, avoiding the use of dextran sulfate sodium. The resulting RC tumors invaded from the mucosal surface and metastasized to distant organs. Histologically, the tumors closely resemble human RC and mirror remodeling of the tumor microenvironment in response to radiation. This murine RC model thus recapitulates key aspects of human RC pathogenesis and presents an accessible approach for more physiologically accurate, preclinical efficacy studies.

Item Type: Paper
Subjects: Investigative techniques and equipment > radiation treatment and equipment
diseases & disorders > cancer > cancer types > colon cancer
diseases & disorders > cancer > cancer types > colon cancer
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Lowe lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 19 December 2022
Date Deposited: 02 Oct 2023 18:20
Last Modified: 27 Nov 2023 14:49
PMCID: PMC9795330
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