The transcriptional and functional properties of mouse epiblast stem cells resemble the anterior primitive streak.

Kojima, Yoji, Kaufman-Francis, Keren, Studdert, Joshua B, Steiner, Kirsten A, Power, Melinda D, Loebel, David AF, Jones, Vanessa, Hor, Angelyn, de Alencastro, Gustavo, Logan, Grant J, Teber, Erdahl T, Tam, Oliver H, Stutz, Michael D, Alexander, Ian E, Pickett, Hilda A, Tam, Patrick PL (January 2014) The transcriptional and functional properties of mouse epiblast stem cells resemble the anterior primitive streak. Cell Stem Cell, 14 (1). pp. 107-120. ISSN 1934-5909

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DOI: 10.1016/j.stem.2013.09.014


Mouse epiblast stem cells (EpiSCs) can be derived from a wide range of developmental stages. To characterize and compare EpiSCs with different origins, we derived a series of EpiSC lines from pregastrula stage to late-bud-stage mouse embryos. We found that the transcriptomes of these cells are hierarchically distinct from those of the embryonic stem cells, induced pluripotent stem cells (iPSCs), and epiblast/ectoderm. The EpiSCs display globally similar gene expression profiles irrespective of the original developmental stage of the source tissue. They are developmentally similar to the ectoderm of the late-gastrula-stage embryo and behave like anterior primitive streak cells when differentiated in vitro and in vivo. The EpiSC lines that we derived can also be categorized based on a correlation between gene expression signature and predisposition to differentiate into particular germ-layer derivatives. Our findings therefore highlight distinct identifying characteristics of EpiSCs and provide a foundation for further examination of EpiSC properties and potential.

Item Type: Paper
Subjects: ?? Animals ??
?? Biomarkers ??
?? Blotting, Western ??
?? Cell Differentiation ??
?? Cell Lineage ??
?? Cell Proliferation ??
?? Cells, Cultured ??
?? Embryo, Mammalian ??
?? Embryonic Stem Cells ??
?? Female ??
?? Fibroblasts ??
?? Gastrulation ??
?? Gene Expression Profiling ??
?? Germ Layers ??
?? Immunoenzyme Techniques ??
?? Mice ??
?? Mice, Inbred C57BL ??
?? Oligonucleotide Array Sequence Analysis ??
?? Pluripotent Stem Cells ??
?? Primitive Streak ??
?? RNA, Messenger ??
?? Real-Time Polymerase Chain Reaction ??
?? Reverse Transcriptase Polymerase Chain Reaction ??
CSHL Authors:
Communities: CSHL labs > Hammell M. lab
SWORD Depositor: CSHL Elements
Depositing User: CSHL Elements
Date: 2 January 2014
Date Deposited: 03 Feb 2021 16:39
Last Modified: 03 Feb 2021 16:39

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