How Escherichia coli is equipped to oxidize hydrogen under different redox conditions

Lukey, M. J., Parkin, A., Roessler, M. M., Murphy, B. J., Harmer, J., Palmer, T., Sargent, F., Armstrong, F. A. (February 2010) How Escherichia coli is equipped to oxidize hydrogen under different redox conditions. J Biol Chem, 285 (6). pp. 3928-38. ISSN 1083-351X (Public Dataset)

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DOI: 10.1074/jbc.M109.067751


The enterobacterium Escherichia coli synthesizes two H(2) uptake enzymes, Hyd-1 and Hyd-2. We show using precise electrochemical kinetic measurements that the properties of Hyd-1 and Hyd-2 contrast strikingly, and may be individually optimized to function under distinct environmental conditions. Hyd-2 is well suited for fast and efficient catalysis in more reducing environments, to the extent that in vitro it behaves as a bidirectional hydrogenase. In contrast, Hyd-1 is active for H(2) oxidation under more oxidizing conditions and cannot function in reverse. Importantly, Hyd-1 is O(2) tolerant and can oxidize H(2) in the presence of air, whereas Hyd-2 is ineffective for H(2) oxidation under aerobic conditions. The results have direct relevance for physiological roles of Hyd-1 and Hyd-2, which are expressed in different phases of growth. The properties that we report suggest distinct technological applications of these contrasting enzymes.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
organism description > bacteria > escherichia coli
CSHL Authors:
Depositing User: Adrian Gomez
Date: 5 February 2010
Date Deposited: 27 Jan 2020 16:42
Last Modified: 27 Jan 2020 16:42
PMCID: PMC2823535
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