A permanent root tip smear method

Warmke, Harry Earl (July 1935) A permanent root tip smear method. Stain Technology, 10 (3). pp. 101-103.

URL: http://www.tandfonline.com/doi/abs/10.3109/1052029...
DOI: 10.3109/10520293509116022


Kill root tips in 1 part glacial acetic acid to 3 parba RB Solute alcohol for 12 or more hours. Remove from king fluid a d place for 5 to 10 minutes in a solution consisting of 1 part 95% alcohol to 1 part concentrated HC1. Transfer to Carnoy's fluid for 5 minutes or longer. Cut a small piece (0.5 mm. or less) off the tip of the root Press directly on the piece of root with a small fiat scalpel; the cells will now separate and float free in the stain. Place cover slip over the drop of stain and apply gentle pressure. Heat carefully by paseing the slide 3 or 4 times thru the flame of an alcohol lamp. Seal with heated mixture of 1 part Parowax to 1 part gum mastie. Make permanent by the McClintock permanent method. and place on a clean slide in a small drop of iron-ace-sinin.

Item Type: Paper
Subjects: Investigative techniques and equipment
Communities: The Carnegie Institution Department of Genetics
Depositing User: Elizabeth Pessala
Date: July 1935
Date Deposited: 12 Jun 2017 20:59
Last Modified: 12 Jun 2017 20:59
URI: https://repository.cshl.edu/id/eprint/35002

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