Similarity of an Estrogen-Induced Protein and a Luteinizing-Hormone Releasing Hormone-Induced Protein

Mobbs, C., Fink, G., Johnson, M., Welch, W., Pfaff, D. (April 1989) Similarity of an Estrogen-Induced Protein and a Luteinizing-Hormone Releasing Hormone-Induced Protein. Molecular and Cellular Endocrinology, 62 (2). pp. 297-306. ISSN 0303-7207

DOI: 10.1016/0303-7207(89)90017-8


Estradiol induces a 70 kDa protein ('EI70') which is synthesized in vivo in the female rat ventromedial hypothalamus (VMH) and transported to the midbrain central gray, suggesting a role for EI70 in the female mating behavior, lordosis. Luteinizing hormone releasing hormone (LHRH), in addition to stimulating gonadotropin release, potentiates pituitary responsiveness to subsequent exposure to LHRH (the 'priming' effect), facilitates lordosis and induces the synthesis of a 70 kDa protein ('LHRH70') in pituitary in vitro. We now report that EI70 precisely co-migrates on two-dimensional (2-D) gels with the pituitary protein induced by LHRH both in vitro and in vivo. Furthermore, both proteins migrate on 2-D gels in the vicinity of a protein recognized after immunoblotting by antibodies to the heat-shock-70 kDa protein family. The induction of a common protein by estrogen or LHRH could represent a common mechanism by which these hormones facilitate secretion, and by which these hormones interact.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > small molecules > estrogen
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > hormones
organs, tissues, organelles, cell types and functions > tissues types and functions > pituitary gland
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: April 1989
Date Deposited: 27 Jun 2017 19:34
Last Modified: 27 Jun 2017 19:34
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