Imaging sialylated tumor cell glycans in vivo

Neves, A. A., Stockmann, H., Harmston, R. R., Pryor, H. J., Alam, I. S., Ireland-Zecchini, H., Lewis, D. Y., Lyons, S. K., Leeper, F. J., Brindle, K. M. (August 2011) Imaging sialylated tumor cell glycans in vivo. FASEB J, 25 (8). pp. 2528-37. ISSN 1530-6860 (Electronic)0892-6638 (Linking)

DOI: 10.1096/fj.10-178590


Cell surface glycans are involved in numerous physiological processes that involve cell-cell interactions and migration, including lymphocyte trafficking and cancer metastasis. We have used a bioorthogonal metabolic labeling strategy to detect cell surface glycans and demonstrate, for the first time, fluorescence and radionuclide imaging of sialylated glycans in a murine tumor model in vivo. Peracetylated azido-labeled N-acetyl-mannosamine, injected intraperitoneally, was used as the metabolic precursor for the biosynthesis of 5-azidoneuraminic, or azidosialic acid. Azidosialic acid-labeled cell surface glycans were then reacted, by Staudinger ligation, with a biotinylated phosphine injected intraperitoneally, and the biotin was detected by subsequent intravenous injection of a fluorescent or radiolabeled avidin derivative. At 24 h after administration of NeutrAvidin, labeled with either a far-red fluorophore or (111)In, there was a significant azido-labeled N-acetyl-mannosamine-dependent increase in tumor-to-tissue contrast, which was detected using optical imaging or single-photon-emission computed tomography (SPECT), respectively. The technique has the potential to translate to the clinic, where, given the prognostic relevance of altered sialic acid expression in cancer, it could be used to monitor disease progression.

Item Type: Paper
Uncontrolled Keywords: Animals Antigens, Tumor-Associated, Carbohydrate/chemistry/metabolism Carcinoma, Lewis Lung/*metabolism Cell Line, Tumor Flow Cytometry Lymphoma, T-Cell/*metabolism Male Mice Mice, Inbred BALB C Mice, Nude Microscopy, Confocal Polysaccharides/chemistry/*metabolism Sialic Acids/chemistry Tomography, Emission-Computed, Single-Photon
Subjects: diseases & disorders > cancer
CSHL Authors:
Communities: CSHL labs > Lyons lab
Depositing User: Matt Covey
Date: August 2011
Date Deposited: 11 May 2017 20:09
Last Modified: 11 May 2017 20:09
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