TCW, J., Carvalho, C. M. B., Yuan, B., Gu, S., Altheimer, A. N., McCarthy, S., Malhotra, D., Sebat, J., Siegel, A. J., Rudolph, U., Lupski, J. R., Levy, D. L., Brennand, K. J. (March 2017) Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis. Stem Cell Reports, 8 (3). pp. 519-528.
Abstract
In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.
| Item Type: | Paper |
|---|---|
| Subjects: | diseases & disorders > mental disorders > dissasosiative disorders diseases & disorders > mental disorders > personality disorders organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells organs, tissues, organelles, cell types and functions > cell types and functions > cell types > stem cells |
| CSHL Authors: | |
| Communities: | CSHL labs > McCombie lab |
| Depositing User: | Matt Covey |
| Date: | 14 March 2017 |
| Date Deposited: | 02 Mar 2017 17:04 |
| Last Modified: | 20 Apr 2017 19:18 |
| PMCID: | PMC5355568 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34134 |
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