Heavy meromyosin labeling of intermediate filaments in cultured connective tissue cells

Buckley, I. K., Raju, T. R., Stewart, M. (September 1978) Heavy meromyosin labeling of intermediate filaments in cultured connective tissue cells. J Cell Biol, 78 (3). pp. 644-52. ISSN 0021-9525 (Print)0021-9525 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/359569
DOI: 10.1083/jcb.78.3.644


Mild treatment with trypsin causes a radical change in the heavy meromyosin (HMM) binding properties of intermediate filaments in glycerinated, myosin-extracted cultured chick embryo connective tissue cells. In non-trypsin-treated cells, HMM labeling of filaments was often indistinct and variable in its distribution. By contrast, in cells treated with trypsin (under conditions which allowed most intermediate filaments to survive), virtually all filaments, including those of intermediate size, decorated with HMM to give distinct arrowhead patterns. We suggest that most intermediate filaments in such cells contain a core of F-actin masked by trypsin-labile accessory proteins.

Item Type: Paper
Uncontrolled Keywords: Actins/*analysis Cells, Cultured Connective Tissue Cells Cytoplasm/*analysis Cytoskeleton/*analysis Myosin Subfragments Trypsin/pharmacology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > myosin
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: September 1978
Date Deposited: 12 Dec 2016 16:43
Last Modified: 12 Dec 2016 16:43
PMCID: PMC2110198
Related URLs:
URI: https://repository.cshl.edu/id/eprint/33317

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