Biological activity of purified simian virus 40 T antigen proteins

Tjian, R., Fey, G., Graessmann, A. (March 1978) Biological activity of purified simian virus 40 T antigen proteins. Proc Natl Acad Sci U S A, 75 (3). pp. 1279-83. ISSN 0027-8424 (Print)0027-8424 (Linking)

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Proteins related to simian virus 40 (SV40) T antigen uere isolated from cells infected with adenovirus 2/SV40 hybrids Ad2+D2 and Ad2+ND1 dp2 as well as from a line of human cells (SV80) transformed by SV40. The 96,000- and 107,000-dalton proteins of SV80 and Ad2+D2, after injection into the cytoplasm of cultured cells, rapidly accumulate in the nuclei, where they remain antigenically reactive for at least 20 hr and trigger DNA synthesis in quiescent cells. By contrast, the 23,000-dalton protein coded by Ad2+ND1 dp2 does not stimulate cellular DNA synthesis. However, all three purified proteins are able to provide helper function for the growth of adenovirus 2 in monkey cells. Thus, purified SV40 T antigen and proteins that share sequences with it retain the ability to carry out at least two functions associated with the product of the A gene of SV40.

Item Type: Paper
Uncontrolled Keywords: Adenoviruses, Human/growth & development Antigens, Neoplasm/*metabolism Antigens, Viral/*metabolism Cell Line Cell Nucleus/metabolism DNA/biosynthesis Genes, Viral Helper Viruses/metabolism Simian virus 40/genetics/immunology/*metabolism Structure-Activity Relationship Viral Proteins/*metabolism
Subjects: organism description > virus > SV40
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: March 1978
Date Deposited: 13 Dec 2016 16:05
Last Modified: 09 Nov 2017 21:04
PMCID: PMC411454
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