Suppression of amber mutants in vitro induced by low temperature

Manley, J. L., Gesteland, R. F. (November 1978) Suppression of amber mutants in vitro induced by low temperature. J Mol Biol, 125 (4). pp. 433-47. ISSN 0022-2836 (Print)0022-2836 (Linking)

DOI: 10.1016/0022-2836(78)90309-1


Amber mutations are efficiently and specifically suppressed during protein synthesis in vitro in an Su− S-30 extract at 25 °C, but not at 37 °C. Eight different amber mutations in three different genes have been tested, and all are suppressed. The efficiencies of suppression range from 20 to 35%, when protein synthesis is at the Mg2+ concentration optimal for β-galactosidase synthesis at 25 °C. The suppression efficiency increases to approximately 60% at higher Mg2+ concentrations, and is reduced to less than 5% at very low concentrations. Ochre and UGA mutations are not suppressed at all under these conditions. The amber suppression is inhibited by addition of a purified protein synthesis release factor to the reaction, or when the protein synthesis reaction takes place in extracts derived from bacteria which are streptomycin-resistant.

Item Type: Paper
Uncontrolled Keywords: Bacterial Proteins/*biosynthesis Codon Cold Temperature Enzyme Repression Magnesium/pharmacology *Mutation Peptide Chain Termination, Translational *Suppression, Genetic beta-Galactosidase/biosynthesis
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification
organism description > bacteria
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 15 November 1978
Date Deposited: 14 Dec 2016 21:02
Last Modified: 14 Dec 2016 21:02
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