Nestin-Based Reporter Transgenic Mouse Lines

Mignone, J., Peunova, N., Enikolopov, G. (July 2016) Nestin-Based Reporter Transgenic Mouse Lines. Methods Mol Biol, 1453. pp. 7-14. ISSN 1940-6029 (Electronic)1064-3745 (Linking)

DOI: 10.1007/978-1-4939-3786-8_2


Nestin expression marks stem and progenitor cells of the neural lineage. Transgenic mouse lines, originally generated to identify neural stem cells, can also help to identify, track, and isolate stem and progenitor cells in a range of tissues of the ectodermal, endodermal, and mesodermal origin. Here, we describe the generation of transgenic mouse lines expressing fluorescent proteins (FP) under the control of critical regulatory elements of the nestin gene and their use for identifying and analyzing adult stem and progenitor cells in various tissues.

Item Type: Paper
Uncontrolled Keywords: Hair-follicle-associated pluripotent (HAP) Immunocytochemistry Nestin Nestin-GFP Reporter lines Stem cells Transgenic animals
Subjects: Investigative techniques and equipment > microscopy > immunoflourescence microscopy
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > nestin
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: Matt Covey
Date: 19 July 2016
Date Deposited: 25 Jul 2016 19:36
Last Modified: 25 Jul 2016 19:36
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