Transformation of rat cells by the hybrid virus Ad2(2+) HEY

Galloway, D. A., Lukanidin, E., Topp, W. C., Sambrook, J. (February 1979) Transformation of rat cells by the hybrid virus Ad2(2+) HEY. J Gen Virol, 42 (2). pp. 339-56. ISSN 0022-1317 (Print)0022-1317 (Linking)

DOI: 10.1099/0022-1317-42-2-339


A set of four isogenic rat cell lines transformed by Ad22+ HEY have been studied. While all of the cell lines synthesize SV40 T antigen, only one expresses adenovirus 2 T antigen: none expresses SV40 V antigen or adenovirus 2 fibre antigen. Three cell lines contain 1 to 2 virus equivalents of SV40 and adenoviral sequences per diploid quantity of rate cell DNA and the fourth line contains five copies of SV40 and 20 copies of the adenovirus genome. At least three of the cell lines contain DNA sequences from the helper adenovirus 2 in addition to sequences from the Ad2+ HEY genome. The patterns of integrated virus sequences are complex suggesting multiple insertions of both adenovirus and SV40 DNA sequences. SV40 can be rescued from three cell lines by fusion with permissive cells.

Item Type: Paper
Uncontrolled Keywords: *Adenoviruses, Human/analysis/immunology Antigens, Neoplasm/analysis Antigens, Viral/analysis Cell Line *Cell Transformation, Neoplastic *Cell Transformation, Viral *DNA, Viral/analysis *Hybridization, Genetic Simian virus 40/analysis/immunology
Subjects: organism description > virus > adenovirus
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell transformation
organism description > virus > SV40
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: February 1979
Date Deposited: 17 May 2016 14:39
Last Modified: 17 May 2016 14:39
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