Identification of a nonprocessive telomerase activity from mouse cells

Prowse, K. R., Avilion, A. A., Greider, C. W. (February 1993) Identification of a nonprocessive telomerase activity from mouse cells. Proc Natl Acad Sci U S A, 90 (4). pp. 1493-7. ISSN 0027-8424 (Print)

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DOI: 10.1073/pnas.90.4.1493


Telomerase activity was identified in extracts from several different mouse cell lines. Addition of telomeric TTAGGG repeats was specific to telomeric oligonucleotide primers and sensitive to pretreatment with RNase A. In contrast to the hundreds of repeats synthesized by the human and Tetrahymena telomerase enzymes in vitro, mouse telomerase synthesized only one or two TTAGGG repeats onto telomeric primers. The products observed after elongation of primers with circularly permuted (TTAGGG)3 sequences and after chain termination with ddATP or ddTTP indicated that mouse telomerase pauses after the addition of the first dG residue in the sequence TTAGGG. The short length of the products synthesized by mouse telomerase was not due to a diffusible inhibitor in the mouse extract, because the human telomerase continued to synthesize long products when mixed with mouse fractions. Primer challenge experiments showed that the human enzyme synthesized long TTAGGG repeats processively in vitro, whereas the mouse telomerase appeared to be much less processive. The identification of short telomerase reaction products in mouse extracts suggests that extracts from other organisms may also generate only short products. This knowledge may aid in the identification of telomerase activity in organisms where activity has not yet been detected.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Cell Line Comparative Study DNA Nucleotidylexotransferase/isolation & purification/ metabolism Humans Mice Molecular Sequence Data Repetitive Sequences, Nucleic Acid Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Species Specificity Substrate Specificity Tetrahymena/enzymology Tumor Cells, Cultured
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > telomerase
CSHL Authors:
Communities: CSHL labs > Greider lab
Depositing User: Matt Covey
Date: 15 February 1993
Date Deposited: 08 Apr 2016 20:00
Last Modified: 09 Nov 2017 20:02
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