Proliferation in vivo and in culture of differentiated adult atrial cardiomyocytes from transgenic mice

Steinhelper, M. E., Lanson, N. A., Dresdner, K. P., Delcarpio, J. B., Wit, A. L., Claycomb, W. C., Field, L. J. (December 1990) Proliferation in vivo and in culture of differentiated adult atrial cardiomyocytes from transgenic mice. Am J Physiol, 259 (6 Pt 2). H1826-34. ISSN 0002-9513 (Print)0002-9513 (Linking)



Transgenic mice expressing atrial natriuretic factor-SV40 T-antigen fusion genes (ANF-TAG) developed unilateral right atrial tumors composed of differentiated dividing cardiomyocytes. The atrial tumors could be propagated as transplantable tumor lineages in syngeneic animals. Cardiomyocytes derived from ANF-TAG atrial tumors did not proliferate in tissue culture. However, cardiomyocytes derived from the transplantable tumor lines proliferated in culture, and these proliferating cardiomyocytes could be passaged in culture and recovered from frozen stocks. Cardiomyocytes from either tumor source were highly differentiated as determined by diverse functional and structural criteria. The cells continued to express numerous cardiac-specific proteins and retained ultrastructural features characteristic of cardiomyocytes including well-formed myofibrils, transverse tubules, and intercalated disks. In addition, the cultured cells displayed spontaneous electrical and contractile activities. These atrial tumor cardiomyocytes are a novel experimental resource for the identification of genes regulating the cardiomyocyte cell cycle.

Item Type: Paper
Uncontrolled Keywords: Animals Antigens, Viral, Tumor/*genetics Atrial Natriuretic Factor/*genetics Cell Differentiation Cell Division Cells, Cultured Cloning, Molecular Culture Techniques Heart Atria Heart Transplantation Mice Mice, Transgenic Myocardium/*pathology Neoplasm Transplantation Phenotype Simian virus 40/immunology Tumor Cells, Cultured
Subjects: Investigative techniques and equipment > cell culture
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell proliferation
organism description > animal > mammal > rodent > mouse
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > transgenic animal
CSHL Authors:
Communities: CSHL labs
CSHL labs > Stillman lab
Depositing User: Matt Covey
Date: December 1990
Date Deposited: 28 Mar 2016 17:01
Last Modified: 28 Mar 2016 17:01
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