Interaction of the v-rel protein with an NF-kappa B DNA binding site

Kabrun, N., Hodgson, J. W., Doemer, M., Mak, G., Franza, B. R., Enrietto, P. J. (March 1991) Interaction of the v-rel protein with an NF-kappa B DNA binding site. Proc Natl Acad Sci U S A, 88 (5). pp. 1783-7. ISSN 0027-8424 (Print)0027-8424 (Linking)

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The avian reticuloendotheliosis virus T contains within its genome the oncogene rel. The expression of this gene is responsible for the induction of lymphoid tumors in birds. Recently, the rel gene was shown to be related to the p50 DNA binding subunit of the transcription factor complex NF-kappa B. Binding sites for the NF-kappa B complex are found in the enhancer regions of a number of genes, including the immunoglobulin kappa gene and the human immunodeficiency virus long terminal repeat. In this communication we identify an activity from avian reticuloendotheliosis virus T-transformed avian lymphoid cells that binds in an electrophoretic-mobility-shift assay to an NF-kappa B binding site from the kappa enhancer. This activity contains proteins immunologically related to rel, as detected by polyclonal and monoclonal antibodies directed against v-rel. In a DNA affinity precipitation assay using the NF-kappa B site from the human immunodeficiency virus long terminal repeat, v-rel and several other proteins were identified. These data suggest that oncogenic transformation by v-rel is the result of an altered pattern of gene expression.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Binding Sites Cell Line Cell Transformation, Neoplastic Chromatography, Affinity Molecular Sequence Data NF-kappa B/genetics/*metabolism Oligonucleotide Probes Oncogene Proteins v-rel Oncogenes Protein-Tyrosine Kinases/metabolism Reticuloendotheliosis virus/*genetics Retroviridae Proteins, Oncogenic/genetics/isolation & purification/*metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
organism description > virus
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 1 March 1991
Date Deposited: 06 Jan 2016 21:57
Last Modified: 09 Nov 2017 20:37
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