SIT4 protein phosphatase is required for the normal accumulation of SWI4, CLN1, CLN2, and HCS26 RNAs during late G1

Fernandez-Sarabia, M. J., Sutton, A., Zhong, T., Arndt, K. T. (December 1992) SIT4 protein phosphatase is required for the normal accumulation of SWI4, CLN1, CLN2, and HCS26 RNAs during late G1. Genes Dev, 6 (12A). pp. 2417-28. ISSN 0890-9369 (Print)0890-9369 (Linking)

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DOI: 10.1101/gad.6.12a.2417


In Saccharomyces cerevisiae, the RNA levels of the G1 cyclins CLN1, CLN2, and HCS26 increase dramatically during the late G1 phase of the cell cycle. The SIT4 gene, which encodes a serine/threonine protein phosphatase, is required for the normal accumulation of CLN1, CLN2, and HCS26 RNAs during late G1. This requirement for SIT4 in normal G1 cyclin RNA accumulation is at least partly via SWI4. Strains containing mutations in SIT4 are sensitive to the loss of either CLN2 or CLN3 function. At the nonpermissive temperature, temperature-sensitive sit4 strains are blocked for both bud emergence and DNA synthesis. Heterologous expression of CLN2 in the absence of SIT4 function results in DNA synthesis, but most of the cells are still blocked for bud emergence. Therefore, SIT4 is required for at least two late G1 or G1/S functions: the normal accumulation of G1 cyclin RNAs (which is required for DNA synthesis) and some additional function that is required for bud emergence or cell cycle progression through late G1 or G1/S.

Item Type: Paper
Uncontrolled Keywords: Cyclins/genetics DNA-Binding Proteins Fungal Proteins/*genetics G1 Phase/*genetics Phosphoprotein Phosphatases/genetics/*metabolism Protein Phosphatase 2 RNA, Fungal/genetics Saccharomyces cerevisiae/cytology/enzymology/*genetics *Saccharomyces cerevisiae Proteins Transcription Factors/genetics
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > protein phosphatase
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: December 1992
Date Deposited: 01 Oct 2015 16:19
Last Modified: 03 Nov 2017 20:44
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