Glutamate receptor binding in juvenile and adult Rana pipiens CNS

Cline, H. T., McDonald, J. W., Constantine-Paton, M. (May 1994) Glutamate receptor binding in juvenile and adult Rana pipiens CNS. J Neurobiol, 25 (5). pp. 488-502.

DOI: 10.1002/neu.480250504


Autoradiographic methods were used to map NMDA- and quisqualate-sensitive glutamate binding sites in the brain of mature and juvenile Rana pipiens frogs. NMDA- and quisqualate-sensitive sites were consistently co-localized in the CNS. The highest glutamate binding occurred in the telencephalon, hypothalamus, and cerebellum. Glutamate binding sites were also specifically localized in visual pathways, including the superficial neuropil of the optic tectum, consistent with glutamate being the retinal ganglion cell neurotransmitter. The distribution of glutamate binding sites in the brain of juvenile postmetamorphic frogs was similar to that in adults. In general, Quis binding increased about twofold in adults compared to juveniles, whereas NMDA binding did not show a comparable developmental increase. To test whether glutamate binding sites are located on retinal axon terminals or on tectal cell dendrites in the optic tectum, juvenile postmetamorphic frogs were enucleated unilaterally, and receptor binding was performed following 1, 3, 7, and 14 days survival. The denervated tectal neuropil showed a delayed decrease in NMDA- and quisqualate-sensitive binding, consistent with the receptors being located on postsynaptic tectal cell dendrites.

Item Type: Paper
Uncontrolled Keywords: Afferent Pathways/physiology Animals Axons/ultrastructure Brain/growth & development/*metabolism Eye Enucleation Horseradish Peroxidase N-Methylaspartate/metabolism Nerve Degeneration/physiology Quisqualic Acid/metabolism Rana pipiens Receptors, Glutamate/*metabolism
Subjects: organs, tissues, organelles, cell types and functions > organs types and functions > brain
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > glutamate receptor
CSHL Authors:
Communities: CSHL labs > Cline lab
Depositing User: Matt Covey
Date: May 1994
Date Deposited: 04 May 2015 21:00
Last Modified: 04 May 2015 21:00
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