Next-Generation Sequencing as Input for Chemometrics in Differential Sensing Routines

Goodwin, S., Gade, A. M., Byrom, M., Herrera, B., Spears, C., Anslyn, E. V., Ellington, A. D. (March 2015) Next-Generation Sequencing as Input for Chemometrics in Differential Sensing Routines. Angew Chem Int Ed Engl, 54 (21). pp. 6339-6342. ISSN 1433-7851

DOI: 10.1002/anie.201501822


Differential sensing (DS) methods traditionally use spatially arrayed receptors and optical signals to create score plots from multivariate data which classify individual analytes or complex mixtures. Herein, a new approach is described, in which nucleic acid sequences and sequence counts are used as the multivariate data without the necessity of a spatial array. To demonstrate this approach to DS, previously selected aptamers, identified from the literature, were used as semi-specific receptors, Next-Gen DNA sequencing was used to generate data, and cell line differentiation was the test-bed application. The study of a principal component analysis loading plot revealed cross-reactivity between the aptamers. The technique generates high-dimensionality score plots, and should be applicable to any mixture of complex and subtly different analytes for which nucleic acid-based receptors exist.

Item Type: Paper
Subjects: Investigative techniques and equipment > assays > next generation sequencing
CSHL Authors:
Communities: CSHL labs > McCombie lab
Depositing User: Matt Covey
Date: 31 March 2015
Date Deposited: 02 Apr 2015 15:37
Last Modified: 19 May 2015 15:56
PMCID: PMC4426058
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