A supressor mutation in Escherichia coli

Witkin, Evelyn M., Kennedy, Frank L. (1951) A supressor mutation in Escherichia coli. The American Naturalist, 85 (821). pp. 141-142.

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Nutritionally deficient mutant strains of bacteria (auxotrophs) are often capable of undergoing "reversion," giving rise to strains having the wild type nutritional state (prototrophs). It is usually impossible to determine whether the prototrophs are true back-mutants, or whether the wild phenotype is restored by the action of an independent suppressor mutation. An auxotroph requiring histidine was isolated from strain B/r of Escherichia coli (which grows well on minimal medium) after ultraviolet irradiation and layering(1). Some time later, the histidineless strain was irradiated with ultraviolet and subjected to penicillin screening(2,3) Among the mutants isolated was one requiring serine or glycine in addition to histidine. This diauxotroph, strain M2, was thus characterized by two growth factor requirements acquired separately and serially, presumably by two mutational steps.

Item Type: Paper
Subjects: organism description > bacteria > escherichia coli
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
CSHL Authors:
Communities: The Carnegie Institution Department of Genetics
Depositing User: Matt Covey
Date: 1951
Date Deposited: 07 Jan 2015 22:21
Last Modified: 03 Sep 2015 19:31
URI: https://repository.cshl.edu/id/eprint/31036

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