Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide

Ermakova, Y. G., Bilan, D. S., Matlashov, M. E., Mishina, N. M., Markvicheva, K. N., Subach, O. M., Subach, F. V., Bogeski, I., Hoth, M., Enikolopov, G., Belousov, V. V. (October 2014) Red fluorescent genetically encoded indicator for intracellular hydrogen peroxide. Nature Communications, 5. p. 5222. ISSN 2041-1723

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DOI: 10.1038/ncomms6222


Reactive oxygen species (ROS) are conserved regulators of numerous cellular functions, and overproduction of ROS is a hallmark of various pathological processes. Genetically encoded fluorescent probes are unique tools to study ROS production in living systems of different scale and complexity. However, the currently available recombinant redox sensors have green emission, which overlaps with the spectra of many other probes. Expanding the spectral range of recombinant in vivo ROS probes would enable multiparametric in vivo ROS detection. Here we present the first genetically encoded red fluorescent sensor for hydrogen peroxide detection, HyPerRed. The performance of this sensor is similar to its green analogues. We demonstrate the utility of the sensor by tracing low concentrations of H2O2 produced in the cytoplasm of cultured cells upon growth factor stimulation. Moreover, using HyPerRed we detect local and transient H2O2 production in the mitochondrial matrix upon inhibition of the endoplasmic reticulum Ca(2+) uptake.

Item Type: Paper
Subjects: physics > biophysics
bioinformatics > genomics and proteomics > small molecules > hydrogen peroxide
diseases & disorders > pulmonary disease > oxidative stress
CSHL Authors:
Communities: CSHL Cancer Center Program > Signal Transduction
CSHL labs > Enikopolov lab
CSHL Cancer Center Shared Resources > Microscopy Service
Depositing User: Matt Covey
Date: 21 October 2014
Date Deposited: 07 Nov 2014 16:10
Last Modified: 16 Jul 2021 18:17
PMCID: PMC4553041
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