The HCF repeat is an unusual proteolytic cleavage signal

Wilson, A. C., Peterson, M. G., Herr, W. (October 1995) The HCF repeat is an unusual proteolytic cleavage signal. Genes Dev, 9 (20). pp. 2445-58. ISSN 0890-9369 (Print)

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DOI: 10.1101/gad.9.20.2445


The herpes simplex virus VP16-associated protein HCF is a nuclear host-cell factor that exists as a family of polypeptides encoded by a single gene. The mature HCF polypeptides are amino- and carboxy-terminal fragments of a large approximately 300-kD precursor protein that arise through cleavage at one or more centrally located sites. The sites of cleavage are the HCF repeats, highly conserved 26-amino-acid sequences repeated six times in the HCF precursor protein. The HCF repeat alone is sufficient to induce cleavage of a heterologous protein, and cleavage occurs at a defined site--PPCE/THET--within the HCF repeat. Alanine-scan mutagenesis was used to identify a large 18-amino-acid segment of the HCF repeat that is important to induce cleavage of a heterologous protein. Even though HCF is cleaved, the majority of amino- and carboxy-terminal cleavage products remain tightly, albeit noncovalently, associated. Modulation of this noncovalent association may provide a mechanism for regulating HCF activity. For example, the cleaved products of an alternative mRNA splicing variant of HCF do not remain associated.

Item Type: Paper
Uncontrolled Keywords: Alternative Splicing Amino Acid Sequence Base Sequence Cell Line Hela Cells Herpes Simplex Virus Protein Vmw65/genetics/ metabolism Host Cell Factor C1 Humans Hydrolysis Molecular Sequence Data Mutation Oligodeoxyribonucleotides Protein Processing, Post-Translational Proteins/genetics/ metabolism RNA, Messenger/genetics Recombinant Proteins/genetics/metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Transcription Factors
Subjects: bioinformatics > genomics and proteomics > design > amino acid design
organism description > virus > herpes simplex virus
organism description > animal > mammal > primates > hominids > human
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Jessica Koos
Date: 15 October 1995
Date Deposited: 11 Aug 2014 20:39
Last Modified: 11 Aug 2014 20:39
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