A novel procedure for pattern analysis of features present on freeze-fractured plasma membranes

Appleyard, S. T., Witkowski, J. A., Ripley, B. D., Shotton, D. M., Dubowitz, V. (March 1985) A novel procedure for pattern analysis of features present on freeze-fractured plasma membranes. Journal of Cell Science, 74. pp. 105-17. ISSN 0021-9533

URL: http://www.ncbi.nlm.nih.gov/pubmed/4030904


We have used statistical methods for the analysis of two-dimensional point patterns to derive quantitative descriptions of the distributions of caveolae on freeze-fractured muscle fibre membranes. One method was based on a quadrat analysis while the second was a new procedure that we have called the interpoint distance analysis. We show that the latter analysis can unambiguously distinguish random, clustered and dispersed patterns and that a single parameter can be derived that can be used to compare different distributions. It is readily applicable to patterns containing several hundred points. Practical details of the method are given and a simple algorithm that can be implemented on a microcomputer is provided. The interpoint distance analysis should prove generally useful in situations where the two-dimensional distribution of objects has to be quantified.

Item Type: Paper
Uncontrolled Keywords: Cell Membrane/ultrastructure Freeze Fracturing Humans Methods Muscles/*ultrastructure Muscular Dystrophies/pathology *Pattern Recognition, Automated Statistics as Topic
Subjects: Investigative techniques and equipment
CSHL Authors:
Communities: Banbury Center
Depositing User: Matt Covey
Date: March 1985
Date Deposited: 01 Dec 2014 19:55
Last Modified: 01 Dec 2014 19:55
Related URLs:
URI: https://repository.cshl.edu/id/eprint/30407

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