Abortive infection by bacteriophage Me1 of Escherichia coli K12 strains bearing the plasmid ColV, I-K94

Reakes, C. F., Gann, A. A., Rossouw, F. T., Rowbury, R. J. (February 1987) Abortive infection by bacteriophage Me1 of Escherichia coli K12 strains bearing the plasmid ColV, I-K94. Journal of General Virology, 68 ( P. pp. 263-72. ISSN 0022-1317 (Print)0022-1317

Abstract

Bacteriophage Me1 is unable to grow on Escherichia coli strains harbouring the ColV,I-K94 plasmid. The nature of this inhibition was investigated, and it was found not to be due to restriction, superinfection exclusion or receptor-mediated resistance, but to be a new example of plasmid-mediated abortive infection. Investigation of events occurring during abortive Me1 infection revealed some differences from previously described cases, especially with regard to late protein synthesis, which did occur, albeit showing abnormal amounts of some proteins. No major differences were observed in membrane permeability of productively and abortively infected cells. Phage-directed DNA synthesis was reduced in abortively infected cells. Comparative studies of Me1 and T4 revealed a striking similarity despite some minor differences.

Item Type:	Paper
Uncontrolled Keywords:	*Bacteriocin Plasmids Cell Membrane Permeability Coliphages/*physiology DNA Replication Escherichia coli/*physiology *Plasmids T-Phages/physiology Viral Proteins/biosynthesis Virus Replication
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organism description > bacteria > escherichia coli bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > plasmid
CSHL Authors:	Gann, Alexander
Communities:	School of Biological Sciences > SBS Administration
Depositing User:	Matt Covey
Date:	February 1987
Date Deposited:	23 Jun 2014 18:39
Last Modified:	09 Sep 2021 18:54
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/30343

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