Nitric oxide regulates cell proliferation during Drosophila development

Kuzin, B., Roberts, I., Peunova, N., Enikolopov, G. (November 1996) Nitric oxide regulates cell proliferation during Drosophila development. Cell, 87 (4). pp. 639-49. ISSN 0092-8674 (Print)

DOI: 10.1016/S0092-8674(00)81384-7


Cell division and subsequent programmed cell death in imaginal discs of Drosophila larvae determine the final size of organs and structures of the adult fly. We show here that nitric oxide (NO) is involved in controlling the size of body structures during Drosophila development. We have found that NO synthase (NOS) is expressed at high levels in developing imaginal discs. Inhibition of NOS in larvae causes hypertrophy of organs and their segments in adult flies, whereas ectopic expression of NOS in larvae has the opposite effect. Blocking apoptosis in eye imaginal discs unmasks surplus cell proliferation and results in an increase in the number of ommatidia and component cells of individual ommatidia. These results argue that NO acts as an antiproliferative agent during Drosophila development, controlling the balance between cell proliferation and cell differentiation.

Item Type: Paper
Uncontrolled Keywords: Animals Apoptosis/drug effects Cell Division/ drug effects DNA/biosynthesis Dihydrolipoamide Dehydrogenase/isolation & purification Drosophila melanogaster/embryology/enzymology/ growth & development Enzyme Inhibitors/pharmacology Extremities/growth & development/pathology Eye/growth & development/ultrastructure Histocytochemistry Hypertrophy Inhibitor of Apoptosis Proteins Larva/enzymology/growth & development Mice Microinjections Nitric Oxide/ pharmacology Nitric Oxide Synthase/antagonists & inhibitors/biosynthesis/genetics Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Transgenes Viral Proteins/biosynthesis/genetics
Subjects: organism description > animal > insect > Drosophila
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell proliferation
bioinformatics > genomics and proteomics > small molecules > nitric oxide
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: Matt Covey
Date: 15 November 1996
Date Deposited: 22 May 2014 15:53
Last Modified: 22 May 2014 15:53
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