Finding a basis for flipping bases

Cheng, X., Blumenthal, R. M. (June 1996) Finding a basis for flipping bases. Structure, 4 (6). pp. 639-45. ISSN 0969-2126 (Print)

DOI: 10.1016/S0969-2126(96)00068-8


Rotation of a DNA nucleotide out of the double helix and into a protein binding pocket ('base flipping') was first observed in the structure of a DNA methyltransferase. There is now evidence that a variety of proteins use base flipping in their interactions with DNA. Though the mechanism for base flipping is still unclear, we propose a three-step pathway: recognizing the target site and increasing the interstrand phosphate-phosphate distance nearby, initiating base flipping by protein invasion of the DNA, and trapping the flipped DNA structure.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence DNA/ chemistry/metabolism Deoxyribonuclease (Pyrimidine Dimer) Endodeoxyribonucleases/chemistry/metabolism Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Nucleotides/metabolism Protein Conformation Proteins/ chemistry Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S. Sequence Alignment Site-Specific DNA Methyltransferase (Cytosine-Specific)/chemistry/metabolism
Subjects: bioinformatics > genomics and proteomics > Mapping and Rendering > DNA Structure Rendering
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 15 June 1996
Date Deposited: 22 May 2014 14:48
Last Modified: 22 May 2014 14:48
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