No change in NMDA receptor-mediated response rise-time during development: evidence against transmitter spillover

Haas, K., Cline, H., Malinow, R. (October 1998) No change in NMDA receptor-mediated response rise-time during development: evidence against transmitter spillover. Neuropharmacology, 37 (10-11). pp. 1393-8. ISSN 0028-3908 (Print)

DOI: 10.1016/S0028-3908(98)00137-3


Glutamatergic transmission was examined in tadpole optic tectum to test the possibility that transmitter concentration reaching N-methyl-D-aspartate (NMDA) receptors increases over development. Pharmacologically isolated NMDA receptor-mediated transmission was monitored with whole-cell recordings. Synaptic responses were recorded from cells at different locations in the optic tectum, corresponding to different stages of development. Rise-times and decay-times of NMDA currents were analyzed. We found no significant correlation between rise-time and developmental stage. As NMDA rise-times can correlate with concentration for glutamate concentrations below 200 microM, these results argue that, if there is developmental variation in transmitter concentration, this occurs for values greater than 200 microM. Furthermore, we found a correlation between rise-times and decay-times, consistent with a model in which transmitter concentration is high and rise-time is controlled by channel closings. These results argue against synaptic models in which low concentrations of transmitter (as by spillover from nearby release sites) selectively activates NMDA receptors.

Item Type: Paper
Uncontrolled Keywords: Animals Comparative Study Larva N-Methylaspartate/ pharmacology Patch-Clamp Techniques Receptors, N-Methyl-D-Aspartate/drug effects/ physiology Superior Colliculus/drug effects/ growth & development Synaptic Transmission/drug effects/ physiology Xenopus
Subjects: bioinformatics > genomics and proteomics > small molecules > NMDA receptor
Investigative techniques and equipment > patch-clamp recording
Investigative techniques and equipment > recording devices > patch-clamp recording
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > synaptic transmission
organism description > animal > Frog > xenopus
CSHL Authors:
Communities: CSHL labs > Cline lab
CSHL labs > Malinow lab
Depositing User: Kathleen Darby
Date: October 1998
Date Deposited: 05 May 2014 13:44
Last Modified: 05 May 2014 13:44
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