Flow cytofluorometric analysis of insulin binding and internalization by Swiss 3T3 cells

Murphy, R. F., Powers, S., Verderame, M., Cantor, C. R., Pollack, R. (May 1982) Flow cytofluorometric analysis of insulin binding and internalization by Swiss 3T3 cells. Cytometry, 2 (6). pp. 402-6. ISSN 0196-4763 (Print)0196-4763

URL: http://www.ncbi.nlm.nih.gov/pubmed/6804197
DOI: 10.1002/cyto.990020608


The binding of a fluorescein-isothiocyanate derivative of insulin to Swiss 3T3 cells was measured by flow cytometry. The kinetics of the subsequent internalization were also measured; at a concentration of 1 microM labeled insulin approximately 25% of the internalization was insulin-specific. The kinetics of endocytosis were contrasted to those of fluorescent derivatives of histone and dextran. In addition, the fusion of endocytic vesicles containing insulin or dextran with lysosomes was detected by measuring the pH-dependent increase in fluorescein fluorescein fluorescence caused by the addition of chloroquine. The application of these results to the analysis of growth control by insulin and related hormones is discussed.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Line Cell Membrane/*metabolism *Endocytosis Flow Cytometry Fluorescein-5-isothiocyanate Fluoresceins Insulin/*metabolism Kinetics Lysosomes/physiology Mice Organoids/physiology Temperature Thiocyanates
Subjects: Investigative techniques and equipment
Investigative techniques and equipment > flow cytometry
organs, tissues, organelles, cell types and functions > organs types and functions > metabolism
CSHL Authors:
Communities: CSHL labs > Powers lab
Depositing User: Matt Covey
Date: May 1982
Date Deposited: 26 Feb 2014 16:48
Last Modified: 26 Feb 2014 16:48
Related URLs:
URI: https://repository.cshl.edu/id/eprint/29509

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