A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae

Kostriken, R., Strathern, J. N., Klar, A. J. S., Hicks, J. B., Heffron, F. (1983) A site-specific endonuclease essential for mating-type switching in Saccharomyces cerevisiae. Cell, 35 (1). pp. 167-174. ISSN 00928674 (ISSN)

URL: http://www.ncbi.nlm.nih.gov/pubmed/6313222


We have detected two site-specific endonucleases in strains of Saccharomyces cerevisiae. One endonuclease, which we call YZ endo, is present only in yeast strains that are undergoing mating-type interconversion. The site at which YZ endo cleaves corresponds to the in vivo double-strand break occurring at the mating-type locus in,yeast undergoing mating-type interconversion. YZ endo generates a site-specific double-strand break having 4-base 3′ extensions terminating in 3′ hydroxyl groups. The site of cleavage occurs in the Z1 region near the YZ junction of the mating-type locus. Mutant mating-type loci known to decrease the frequency of mating-type interconversion are correspondingly poor substrates for YZ endo in vitro. In vitro analysis of a number of such altered recognition sites has delimited the sequences required for cleavage. The molecular genetics of mating-type interconversion is discussed in the context of this endonucleolytic activity. The second endonuclease, which we refer to as Sce II, is present in all strains of S. cerevisiae we have examined. The cleavage site of Sce II has been determined and proves to be unrelated to the cleavage site of YZ endo. © 1983.

Item Type: Paper
Uncontrolled Keywords: endonuclease conjugation fungus heredity mating type nonhuman saccharomyces cerevisiae Base Sequence Crosses, Genetic Deoxyribonucleases, Type II Site-Specific DNA Restriction Enzymes DNA, Fungal Endodeoxyribonucleases Genes, Fungal Genes, Mating Type, Fungal Mutation Fungi Saccaromyces
Subjects: organism description > yeast
CSHL Authors:
Communities: CSHL labs > Hicks lab
Depositing User: Matt Covey
Date: 1983
Date Deposited: 09 Aug 2013 20:56
Last Modified: 12 Aug 2013 14:23
Related URLs:
URI: https://repository.cshl.edu/id/eprint/28485

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