Sensorimotor assessment of the unilateral 6-hydroxydopamine mouse model of Parkinson's disease

Glajch, K. E., Fleming, S. M., Surmeier, D. J., Osten, P. (2012) Sensorimotor assessment of the unilateral 6-hydroxydopamine mouse model of Parkinson's disease. Behavioural Brain Research, 230 (2). pp. 309-316. ISSN 01664328 (ISSN)

DOI: 10.1016/j.bbr.2011.12.007


Parkinson's disease (PD), the second most common neurodegenerative disorder, is characterized by marked impairments in motor function caused by the progressive loss of dopaminergic neurons in the substantia nigra pars compacta (SNc). Animal models of PD have traditionally been based on toxins, such as 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), that selectively lesion dopaminergic neurons. Motor impairments from 6-OHDA lesions of SNc neurons are well characterized in rats, but much less work has been done in mice. In this study, we compare the effectiveness of a series of drug-free behavioral tests in assessing sensorimotor impairments in the unilateral 6-OHDA mouse model, including six tests used for the first time in this PD mouse model (the automated treadmill " DigiGait" test, the challenging beam test, the adhesive removal test, the pole test, the adjusting steps test, and the test of spontaneous activity) and two tests used previously in 6-OHDA-lesioned mice (the limb-use asymmetry " cylinder" test and the manual gait test). We demonstrate that the limb-use asymmetry, challenging beam, pole, adjusting steps, and spontaneous activity tests are all highly robust assays for detecting sensorimotor impairments in the 6-OHDA mouse model. We also discuss the use of the behavioral tests for specific experimental objectives, such as simple screening for well-lesioned mice in studies of PD cellular pathophysiology or comprehensive behavioral analysis in preclinical therapeutic testing using a battery of sensorimotor tests. © 2011 Elsevier B.V.

Item Type: Paper
Uncontrolled Keywords: 6-OHDA Behavioral test Medial forebrain bundle Mouse Parkinson's disease Sensorimotor impairment 1,2,3,6 tetrahydro 1 methyl 4 phenylpyridine oxidopamine adhesive removal test adjusting steps test animal behavior animal experiment animal model article automated treadmill gait test behavioral research brain electrophysiology challenging beam test comparative effectiveness controlled study dopaminergic nerve cell electric battery experimental test forefoot limb use asymmetry test male manual gait analysis motor coordination motor dysfunction motor performance nigroneostriatal system nonhuman Parkinson disease pathophysiology pole test priority journal sensorimotor function sensory dysfunction spontaneous activity test substantia nigra pars compacta Adrenergic Agents Animals Disease Models, Animal Dopaminergic Neurons Gait Disorders, Neurologic Mice Mice, Inbred C57BL Parkinsonian Disorders Psychomotor Disorders Psychomotor Performance Substantia Nigra
Subjects: diseases & disorders
diseases & disorders > nervous system diseases and disorders
diseases & disorders > nervous system diseases and disorders > Parkinson's disease
organism description > model organism
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Osten lab
Highlight: Osten, Pavel
Depositing User: Matt Covey
Date: 2012
Date Deposited: 04 Apr 2013 21:06
Last Modified: 18 Nov 2014 16:30
PMCID: PMC3324279
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