Sulf-2, a proangiogenic heparan sulfate endosulfatase, is upregulated in breast cancer

Morimoto-Tomita, M., Uchimura, K., Bistrup, A., Lum, D. H., Egeblad, M., Boudreau, N., Werb, Z., Rosen, S. D. (2005) Sulf-2, a proangiogenic heparan sulfate endosulfatase, is upregulated in breast cancer. Neoplasia, 7 (11). pp. 1001-1010. ISSN 15228002 (ISSN)

DOI: 10.1593/neo.05496


Sulf-2 is an endosulfatase with activity against glucosamine-6-sulfate modifications within subregions of intact heparin. The enzyme has the potential to modify the sulfation status of extracellular heparan sulfate proteoglycan (HSPG) glycosaminoglycan chains and thereby to regulate interactions with HSPG-binding proteins. In the present investigation, data mining from published studies was employed to establish Sulf-2 mRNA upregulation in human breast cancer. We further found that cultured breast carcinoma cells expressed Sulf-2 mRNA and released enzymatically active proteins into conditioned medium. In two mouse models of mammary carcinoma, Sulf-2 mRNA was upregulated in comparison to its expression in normal mammary gland. Although mRNA was present in normal tissues, Sulf-2 protein was undetectable; it was, however, detected in some premalignant lesions and in tumors. The protein was localized to the epithelial cells of the tumors. In support of the possible mechanistic relevance of Sulf-2 upregulation in tumors, purified recombinant Sulf-2 promoted angiogenesis in the chick chorioallantoic membrane assay. Copyright © 2005 Neoplasia Press, Inc. All rights reserved.

Item Type: Paper
Uncontrolled Keywords: Angiogenesis Breast cancer Extracellular endosulfatase Heparan sulfate Sulf-2 messenger RNA sulf 2 enzyme sulfatase unclassified drug heparan sulfatase primer DNA SULF2 protein, human sulfotransferase animal experiment animal model animal tissue article breast carcinoma breast epithelium cancer cell culture controlled study culture medium enzyme regulation female human human cell mammary gland mouse nonhuman priority journal protein analysis protein expression protein localization upregulation amino acid sequence animal breast tumor chick embryo chorion enzymology fetus membrane gene expression regulation genetics methodology molecular genetics nucleotide sequence polymerase chain reaction vascularization Allantois Animals Base Sequence Breast Neoplasms DNA Primers Gene Expression Regulation, Enzymologic Gene Expression Regulation, Neoplastic Humans Molecular Sequence Data Neovascularization, Physiologic RNA, Messenger Sulfatases Sulfotransferases
Subjects: diseases & disorders > cancer
diseases & disorders
diseases & disorders > cancer > cancer types > breast cancer
diseases & disorders > cancer > cancer types
CSHL Authors:
Communities: CSHL labs > Egeblad lab
Depositing User: Matt Covey
Date: 2005
Date Deposited: 14 Mar 2013 19:49
Last Modified: 14 Mar 2013 19:49
PMCID: PMC1502017
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