Dendritic Spine Morphology Determines Membrane-Associated Protein Exchange between Dendritic Shafts and Spine Heads

Hugel, S., Abegg, M., De Paola, V., Caroni, P., Gahwiler, B. H., McKinney, R. A. (March 2009) Dendritic Spine Morphology Determines Membrane-Associated Protein Exchange between Dendritic Shafts and Spine Heads. Cerebral Cortex, 19 (3). pp. 697-702. ISSN 1047-3211

Abstract

The purpose of this study was to examine whether variability in the shape of dendritic spines affects protein movement within the plasma membrane. Using a combination of confocal microscopy and the fluorescence loss in photobleaching technique in living hippocampal CA1 pyramidal neurons expressing membrane-linked GFP, we observed a clear correlation between spine shape parameters and the diffusion and compartmentalization of membrane-associated proteins. The kinetics of membrane-linked GFP exchange between the dendritic shaft and the spine head compartment were slower in dendritic spines with long necks and/or large heads than in those with short necks and/or small heads. Furthermore, when the spine area was reduced by eliciting epileptiform activity, the kinetics of protein exchange between the spine compartments exhibited a concomitant decrease. As synaptic plasticity is considered to involve the dynamic flux by lateral diffusion of membrane-bound proteins into and out of the synapse, our data suggest that spine shape represents an important parameter in the susceptibility of synapses to undergo plastic change.

Item Type:	Paper
Uncontrolled Keywords:	compartmentalization FLIP lateral diffusion photobleaching plasticity LONG-TERM POTENTIATION AMPA RECEPTOR TRAFFICKING DIFFUSION PLASTICITY SYNAPSES ACTIVATION STABILITY DYNAMICS GEOMETRY NECK
Subjects:	organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions > cell types organs, tissues, organelles, cell types and functions > cell types and functions organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines organs, tissues, organelles, cell types and functions > cell types and functions > cell types > dendritic cells > dendritic spines organs, tissues, organelles, cell types and functions
CSHL Authors:	De Paola, Vincenzo
Communities:	CSHL labs > Svoboda lab
Depositing User:	Matt Covey
Date:	March 2009
Date Deposited:	21 Feb 2013 17:18
Last Modified:	21 Feb 2013 17:18
Related URLs:	Publisher
URI:	https://repository.cshl.edu/id/eprint/27356

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