Genome-wide mapping indicates that p73 and p63 Co-occupy target sites and have similar DNA-binding profiles in vivo

Yang, A., Zhu, Z., Kettenbach, A., Kapranov, P., McKeon, F., Gingeras, T. R., Struhl, K. (2010) Genome-wide mapping indicates that p73 and p63 Co-occupy target sites and have similar DNA-binding profiles in vivo. PLoS ONE, 5 (7). ISSN 19326203 (ISSN)

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DOI: 10.1371/journal.pone.0011572


Background: The p53 homologs, p63 and p73, share, ~85% amino acid identity in their DNA-binding domains, but they have distinct biological functions. Principal Findings: Using chromatin immunoprecipitation and high-resolution tiling arrays covering the human genome, we identify p73 DNA binding sites on a genome-wide level in ME180 human cervical carcinoma cells. Strikingly, the p73 binding profile is indistinguishable from the previously described binding profile for p63 in the same cells. Moreover, the p73:p63 binding ratio is similar at all genomic loci tested, suggesting that there are few, if any, targets that are specific for one of these factors. As assayed by sequential chromatin immunoprecipitation, p63 and p73 co-occupy DNA target sites in vivo, suggesting that p63 and p73 bind primarily as heterotetrameric complexes in ME180 cells. Conclusions: The observation that p63 and p73 associate with the same genomic targets suggest that their distinct biological functions are due to cell-type specific expression and/or protein domains that involve functions other than DNA binding. © 2010 Yang et al.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: protein p63 protein p73 article binding site carcinoma cell chromatin immunoprecipitation controlled study female gene locus gene mapping gene sequence gene targeting human human cell mouse nonhuman protein DNA binding protein expression rat uterine cervix carcinoma
Subjects: diseases & disorders > cancer
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 2010
Date Deposited: 08 Mar 2012 14:50
Last Modified: 12 Jul 2013 18:15
PMCID: PMC2904373
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