CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells

Liu, W., Putnam, A. L., Xu-yu, Z., Szot, G. L., Lee, M. R., Zhu, S., Gottlieb, P. A., Kapranov, P., Gingeras, T. R., De St. Groth, B. F., Clayberger, C., Soper, D. M., Ziegler, S. F., Bluestone, J. A. (2006) CD127 expression inversely correlates with FoxP3 and suppressive function of human CD4+ T reg cells. Journal of Experimental Medicine, 203 (7). pp. 1701-1711. ISSN 00221007 (ISSN)

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DOI: 10.1084/jem.20060772


Regulatory T (T reg) cells are critical regulators of immune tolerance. Most T reg cells are defined based on expression of CD4, CD25, and the transcription factor, FoxP3. However, these markers have proven problematic for uniquely defining this specialized T cell subset in humans. We found that the IL-7 receptor (CD127) is down-regulated on a subset of CD4+ T cells in peripheral blood. We demonstrate that the majority of these cells are FoxP3+, including those that express low levels or no CD25. A combination of CD4, CD25, and CD127 resulted in a highly purified population of T reg cells accounting for significantly more cells that previously identified based on other cell surface markers. These cells were highly suppressive in functional suppressor assays. In fact, cells separated based solely on CD4 and CD127 expression were anergic and, although representing at least three times the number of cells (including both CD25+CD4+ and CD25-CD4+ T cell subsets), were as suppressive as the "classic" CD4+CD25hi T reg cell subset. Finally, we show that CD127 can be used to quantitate T reg cell subsets in individuals with type 1 diabetes supporting the use of CD127 as a biomarker for human T reg cells. JEM © The Rockefeller University Press.

Item Type: Paper
Uncontrolled Keywords: CD4 antigen interleukin 2 receptor alpha interleukin 7 receptor transcription factor FOXP3 adolescent adult article CD4+ T lymphocyte clinical article down regulation human priority journal protein expression protein protein interaction Biological Markers CD4 Lymphocyte Count Cells, Cultured Diabetes Mellitus, Type 1 Forkhead Transcription Factors Humans Immunologic Memory Middle Aged Receptors, Interleukin-2 Receptors, Interleukin-7 T-Lymphocytes, Regulatory
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein receptor
CSHL Authors:
Communities: CSHL labs > Gingeras lab
Depositing User: CSHL Librarian
Date: 2006
Date Deposited: 08 Mar 2012 17:04
Last Modified: 12 Jul 2013 20:31
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