Nucleotide sequences from the adenovirus-2 genome

Gingeras, T. R., Sciaky, D., Gelinas, R. E., Bing-Dong, J., Yen, C. E., Kelly, M. M., Bullock, P. A., Parsons, B. L., O'Neill, K., Roberts, R. J. (1982) Nucleotide sequences from the adenovirus-2 genome. Journal of Biological Chemistry, 257 (22). pp. 13475-13491. ISSN 00219258 (ISSN)



The sequence of 15,441 nucleotides from the adenovirus-2 genome has been determinend and includes the regions between coordinates 0-32% and 89-100%. These regions contain the early (E) transcription units E1A, E1B, E2B, and E4, the genes for polypeptides IVa2 and IX, the COOH terminus of fiber polypeptide, as well as the two virus-associated RNAs and the leader sequences for the major late mRNAs. Analysis of tryptic peptides from the terminal protein and its precursor has allowed the gene for the precursor terminal protein to be positioned between coordinates 28.9 and 23.5 on the 1-strand. A minimum M(r) = 74,500 is predicted. A second, longer open reading frame is also found on the 1-strand between coordinates 22.9 and 14.2 and predicts a polypeptide of at least M(r) = 120,000. Many open reading frames longer than 10,000 exist within this sequence although less than half of them can be assigned to previously characterized polypeptides. As with other viral genomes, the available coding information is highly compressed. Intergenic distances are very short and examples are found of genes which overlap either on the same strand or the complementary strand.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: radioisotope virus dna adenovirus 2 cell culture human cell in vitro study nonhuman nucleotide sequence Adenoviruses Human Base Sequence Cell Line Cloning, Molecular DNA Viral Genes Structural Genes Viral Hela Cells Human Molecular Weight Peptide Fragments RNA, Messenger Transcription, Genetic Viral Proteins
Subjects: bioinformatics > genomics and proteomics > annotation > sequence annotation
organism description > virus
CSHL Authors:
Communities: CSHL labs > Gingeras lab
CSHL labs > Roberts lab
Depositing User: CSHL Librarian
Date: 1982
Date Deposited: 14 Mar 2012 14:49
Last Modified: 31 Mar 2014 14:25
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