CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication

Zou, L., Mitchell, J., Stillman, B. (February 1997) CDC45, a novel yeast gene that functions with the origin recognition complex and Mcm proteins in initiation of DNA replication. Molecular and Cellular Biology, 17 (2). pp. 553-63. ISSN 0270-7306



The CDC45 gene of Saccharomyces cerevisiae was isolated by complementation of the cold-sensitive cdc45-1 mutant and shown to be essential for cell viability. Although CDC45 genetically interacts with a group of MCM genes (CDC46, CDC47, and CDC54), the predicted sequence of its protein product reveals no significant sequence similarity to any known Mcm family member. Further genetic characterization of the cdc45-1 mutant demonstrated that it is synthetically lethal with orc2-1, mcm2-1, and mcm3-1. These results not only reveal a functional connection between the origin recognition complex (ORC) and Cdc45p but also extend the CDC45-MCM genetic interaction to all known MCM family members that were shown to be involved in replication initiation. Initiation of DNA replication in cdc45-1 cells was defective, causing a delayed entry into S phase at the nonpermissive temperature, as well as a high plasmid loss rate which could be suppressed by tandem copies of replication origins. Furthermore, two-dimensional gels directly showed that chromosomal origins fired less frequently in cdc45-1 cells at the nonpermissive temperature. These findings suggest that Cdc45p, ORC, and Mcm proteins act in concert for replication initiation throughout the genome.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Carrier Proteins genetics Cell Cycle Proteins physiology Cloning Molecular Crosses Genetic DNA Replication physiology DNA Fungal biosynthesis DNA-Binding Proteins Genes, Fungal genetics Genes Lethal Molecular Sequence Data Mutation Nuclear Proteins genetics Peptides pharmacology Plasmids metabolism Replication Origin genetics Restriction Mapping S Phase physiology Saccharomyces cerevisiae Proteins Temperature
Subjects: bioinformatics > genomics and proteomics > annotation > gene expression profiling annotation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: February 1997
Date Deposited: 17 Feb 2012 15:16
Last Modified: 20 Jun 2017 19:35
PMCID: PMC231780
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