Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4

Verreault, A., Kaufman, P. D., Kobayashi, R., Stillman, B. (October 1996) Nucleosome assembly by a complex of CAF-1 and acetylated histones H3/H4. Cell, 87 (1). pp. 95-104. ISSN 0092-8674 (Print)

DOI: 10.1016/S0092-8674(00)81326-4


Chromatin assembly factor 1 (CAF-1) assembles nucleosomes in a replication-dependent manner. The small subunit of CAF-1 (p48) is a member of a highly conserved subfamily of WD-repeat proteins. There are at least two members of this subfamily in both human (p46 and p48) and yeast cells (Hat2p, a subunit of the B-type H4 acetyltransferase, and Msi1p). Human p48 can bind to histone H4 in the absence of CAF-1 p150 and p60. p48, also a known subunit of a histone deacetylase, copurifies with a chromatin assembly complex (CAC), which contains the three subunits of CAF-1 (p150, p60, p48) and H3 and H4, and promotes DNA replication-dependent chromatin assembly. CAC histone H4 exhibits a novel pattern of lysine acetylation that overlaps with, but is distinct from, that reported for newly synthesized H4 isolated from nascent chromatin. Our data suggest that CAC is a key intermediate of the de novo nucleosome assembly pathway and that the p48 subunit participates in other aspects of histone metabolism.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Acetylation Acetyltransferases metabolism Amino Acid Sequence Cell Extracts Cell Line Cell Nucleus Chromatin metabolism Chromosomal Proteins Non-Histone Cloning Molecular Cytoplasm DNA Replication physiology DNA Complementary genetics DNA Binding Proteins chemistry genetics isolation & purification metabolism Histone Acetyltransferases Histones chemistry isolation & purification metabolism Humans Lysine metabolism Molecular Sequence Data Molecular Weight Nucleosomes metabolism Saccharomyces cerevisiae Proteins
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
CSHL Authors:
Communities: CSHL labs > Kobayashi lab
CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: 4 October 1996
Date Deposited: 17 Feb 2012 20:24
Last Modified: 20 Jun 2017 19:37
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