Interaction of a Nuclear Factor with the Polyomavirus Enhancer Region

Ostapchuk, P., Diffley, J. F. X., Bruder, J. T., Stillman, B., Levine, A. J., Hearing, P. (November 1986) Interaction of a Nuclear Factor with the Polyomavirus Enhancer Region. Proceedings of the National Academy of Sciences of the United States of America, 83 (22). pp. 8550-8554. ISSN 0027-8424

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We have identified a factor present in nuclear extracts of undifferentiated F9 murine embryonal carcinoma cells that specifically interacts with the polyomavirus enhancer region. Nuclease "footprint" analysis was used to define the binding site that corresponds precisely to the boundaries of polyoma enhancer element C defined by Veldman et al. [Veldman, G. M., Lupton, S. & Kamen, R. (1985) Mol. Cell. Biol. 5, 649-658] that is required as an enhancer for efficient viral DNA replication and early and late region transcription. The region of nuclease protection contains a 6-base-pair inverted repeat, separated by 3 base pairs, and symmetrical flanking DNase I hypersensitive cleavage sites, suggesting that this factor may bind as a dimer. A cloned 29-base-pair polyoma DNA fragment contains an intact binding domain. Similar levels of binding activity were found in nuclear extracts prepared from differentiated murine F9 cells, as well as murine L cells and human HeLa cells. The factor has been termed "EF-C" for enhancer binding factor to polyoma element C.

Item Type: Paper
Subjects: organism description > virus
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: November 1986
Date Deposited: 02 Mar 2012 19:19
Last Modified: 30 Sep 2019 19:42
PMCID: PMC386968
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