Regulation of SV40 DNA replication by phosphorylation of T antigen

Mohr, I. J., Stillman, B., Gluzman, Y. (January 1987) Regulation of SV40 DNA replication by phosphorylation of T antigen. Embo J, 6 (1). pp. 153-60. ISSN 0261-4189 (Print)



The role of phosphorylation in regulating the biochemical properties of SV40 large T antigen has been examined. Treatment of purified T antigen with calf intestinal alkaline phosphatase resulted in the removal of 80% of the 32P label. This partially dephosphorylated T antigen displayed an increase in its ability to support DNA replication in vitro. This increase in replication activity was paralleled by an activation of specific DNA binding to site II, a necessary element within the origin of SV40 DNA replication. In contrast, the ATPase activity of dephosphorylated T antigen remained unchanged. These results demonstrate that DNA replication is regulated by phosphorylation of an origin specific DNA binding protein.

Item Type: Paper
Additional Information:
Uncontrolled Keywords: Adenosine Triphosphatases metabolism Alkaline Phosphatase/metabolism Antigens Polyomavirus Transforming Antigens Viral Tumor metabolism Cell Line DNA Replication Enzyme Activation Hela Cells metabolism Homeostasis Humans Kinetics Oncogene Proteins Viral metabolism Phosphorylation Protein Kinases metabolism Simian virus 40 genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > phosphorylation
CSHL Authors:
Communities: CSHL labs > Stillman lab
Highlight: Stillman, Bruce W.
Depositing User: CSHL Librarian
Date: January 1987
Date Deposited: 06 Mar 2012 14:10
Last Modified: 20 Jun 2017 20:33
PMCID: PMC553371

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