Whole-cell recording in vivo

DeWeese, M. R. (January 2007) Whole-cell recording in vivo. Curr Protoc Neurosci (chap 6). Unit 6.22. ISSN 1934-8576 (Electronic)

URL: https://www.ncbi.nlm.nih.gov/pubmed/18428661
DOI: 10.1002/0471142301.ns0622s38


In vivo whole-cell patch-clamp recording provides a means for measuring membrane currents and potentials from individual cells in the intact animal. Patch-clamp methods have largely been developed in vitro. This body of work has contributed enormously to the understanding of many important phenomena in excitable cells--including synaptic plasticity in the mammalian central nervous system, and the behavior of individual protein channels. In recent years, an increasing number of groups have applied whole-cell recording techniques in the intact animal. Such in vivo studies offer the tantalizing possibility of uncovering the underlying principles and mechanisms of neural interactions within the natural context of fully intact biological networks. This unit focuses on strategies for overcoming the specific technical challenges posed by in vivo whole-cell recording. A straightforward procedure is described for obtaining whole-cell records from the cortex of the anesthetized rat; this procedure has also been applied successfully to awake animals and other rodent species with minor modifications.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions
organs, tissues, organelles, cell types and functions > tissues types and functions > membranes
Investigative techniques and equipment > patch-clamp recording
Investigative techniques and equipment > recording devices > patch-clamp recording
CSHL Authors:
Communities: CSHL labs > Zador lab
Depositing User: CSHL Librarian
Date: January 2007
Date Deposited: 30 Nov 2011 20:23
Last Modified: 22 Mar 2018 16:28
Related URLs:
URI: https://repository.cshl.edu/id/eprint/22998

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving