Long-term live imaging of neuronal circuits in organotypic hippocampal slice cultures

Gogolla, N., Galimberti, I., DePaola, V., Caroni, P. (September 2006) Long-term live imaging of neuronal circuits in organotypic hippocampal slice cultures. Nature Protocols, 1 (3). pp. 1223-1226. ISSN 1745-2473

URL: https://www.ncbi.nlm.nih.gov/pubmed/17406405
DOI: 10.1038/nprot.2006.169


This protocol details a method for imaging organotypic slice cultures from the mouse hippocampus. The cultures are based on the interface method, which does not require special equipment, is easy to execute, and yields slice cultures that can be imaged repeatedly after they are isolated on postnatal day 6–9 and for up to 6 months in vitro. The preserved tissue architecture facilitates the analysis of defined hippocampal synapses, cells and entire projections. Time-lapse imaging is based on transgenes expressed in the mice, or on constructs introduced through transfection or viral vectors; it can reveal processes that develop over time periods ranging from seconds to months. Imaging can be repeated at least eight times without detectable morphological damage to neurons. Subsequent to imaging, the slices can be processed for immunocytochemistry or electron microscopy, to collect further information about the structures that have been imaged. This protocol can be completed in 35 min.

Item Type: Paper
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > hippocampus
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Svoboda lab
Depositing User: CSHL Librarian
Date: September 2006
Date Deposited: 14 Dec 2011 16:24
Last Modified: 13 Apr 2018 16:53
Related URLs:
URI: https://repository.cshl.edu/id/eprint/22799

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