Phenotypic and functional characterization of adult brain neuropoiesis

Scheffler, B., Walton, N. M., Lin, D. D., Goetz, A. K., Enikolopov, G., Roper, S. N., Steindler, D. A. (June 2005) Phenotypic and functional characterization of adult brain neuropoiesis. Proceedings of the National Academy of Sciences of the United States of America, 102 (26). pp. 9353-9358. ISSN 0027-8424

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DOI: 10.1073/pnas.0503965102


The modern concept of neurogenesis in the adult brain is predicated on the premise that multipotent glial cells give rise to new neurons throughout life. Although extensive evidence exists indicating that this is the case, the transition from glial to neuronal phenotype remains poorly understood. A unique monolayer cell-culture system was developed to induce, expose, and recapitulate the entire developmental series of events of subventricular zone (SVZ) neurogenesis. We show here, using immunophentoypic, ultrastructural, electrophysiological, and time-lapse analyses, that SVZ-derived glial fibrillary acidic protein(low)/A2B5(+)/nestin(+) candidate founder cells undergo metamorphosis to eventually generate large numbers of fully differentiated interneuron phenotypes. A model of postnatal neurogenesis is considered in light of known embryonic events and reveals a limited developmental potential of SVZ stem/progenitor cells, whereby ancestral cells in both embryonic and postnatal/adult settings give rise to glia and GABAergic interneurons.

Item Type: Paper
Uncontrolled Keywords: adult stem cells electrophysiology in vitro neurogenesis subventricular zone neural stem cells subventricular zone progenitor cells mammalian brain subependymal cells precurser cells olfactory bulb mouse brain in-vitro neurons SVZ
Subjects: organs, tissues, organelles, cell types and functions > tissues types and functions > brain subventricular zone
Investigative techniques and equipment > cell culture
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: CSHL Librarian
Date: June 2005
Date Deposited: 05 Jan 2012 16:33
Last Modified: 11 Jan 2018 19:47
PMCID: PMC1150897
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