Tracking topoisomerase activity at the single-molecule level

Charvin, G., Strick, T. R., Bensimon, D., Croquette, V. (2005) Tracking topoisomerase activity at the single-molecule level. Annual Review of Biophysics and Biomolecular Structure, 34. pp. 201-219. ISSN 1056-8700

DOI: 10.1146/annurev.biophys.34.040204.144433


The recent development of new techniques to manipulate single DNA molecules has opened new opportunities for the study of the enzymes that control DNA topology: the type I and II topoisomerases. These single-molecule assays provide a unique way to study the uncoiling of single supercoiled DNA molecules and the unlinking of two intertwined DNAs. They allow for a detailed characterization of the activity of topoisomerases, including the processivity, the chiral discrimination, and the dependence of their enzymatic rate on ATP concentration, degree of supercoiling, and the tension in the molecule. These results shed new light on the mechanism of these enzymes and their function in vivo.

Item Type: Paper
Uncontrolled Keywords: molecular motors DNA replication micromanipulation magnetic tweezers SUPERCOILED DNA MOLECULE supercoiled DNA molecule STEADY-STATE ANALYSIS steady state analysis ESCHERICHIA-COLI escherichia-coli II TOPOISOMERASES topiosomerases DROSOPHILA-MELANOGASTER drosophila melanogaster Drosophila Melanogaster 2-GATE MECHANISM 2-gate mechanism DUPLEX DNA duplex DNA ELASTICITY elasticity TRANSPORT transport FORCE
Subjects: bioinformatics > genomics and proteomics > small molecules > ATP
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sDNA
CSHL Authors:
Communities: CSHL labs > Strick lab
Depositing User: CSHL Librarian
Date: 2005
Date Deposited: 13 Jan 2012 19:41
Last Modified: 02 May 2018 19:36
Related URLs:

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving