The p400 E1A-associated protein is a novel component of the p53 -> p21 senescence pathway

Chan, H. M., Narita, M., Lowe, S. W., Livingstone, D. M. (January 2005) The p400 E1A-associated protein is a novel component of the p53 -> p21 senescence pathway. Genes Dev, 19 (2). pp. 196-201. ISSN 0890-9369

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DOI: 10.1101/gad.1280205


Adenovirus E1A-associated p400 belongs to the SW12/ SNF2 family of chromatin remodeling proteins. Here, we report that p400 is a component of the p53-p21(WAF1/CIP1/sid1) pathway, regulating the p21 transcription and senescence induction program. Acute depletion of p400 expression by shRNA (short hairpin RNA) synthesis led to premature senescence of untransformed human fibroblasts, whose features include G1 arrest, p21 induction, senescence-associated heterochromatic foci (SAHF), and beta-gal staining. Importantly, p400shRNA-induced premature senescence phenotypes were rescued by coexpression of p53-shRNA or p21shRNA. Furthermore, p400 complex colocalized with p53 on the p21 promoter. These data suggest that the p400 complex inhibits p53 --> p21 transcription and the development of premature senescence.

Item Type: Paper
Uncontrolled Keywords: p400 senescence p53 p21 ElA myc HISTONE ACETYLTRANSFERASE COMPLEX histome acetyltransferase complex DNA-BINDING DNA binding CELLULAR SENESCENCE cellular senescence TUMOR SUPPRESSION tumor suppression ONCOGENIC RAS oncogenetic RAS HUMAN-CELLS human cells P53 CHROMATIN chromatin MYC IDENTIFICATION identification WAF1 CIP1 sid1
Subjects: organism description > virus > adeno associated virus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
CSHL Authors:
Communities: CSHL labs > Lowe lab
Depositing User: CSHL Librarian
Date: January 2005
Date Deposited: 13 Jan 2012 19:34
Last Modified: 02 May 2018 19:34
PMCID: PMC545875
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