Nitric oxide is a regulator of hematopoietic stem cell activity

Michurina, T., Krasnov, P., Balazs, A., Nakaya, N., Vasilieva, T., Kuzin, B., Khrushchov, N., Mulligan, R. C., Enikolopov, G. N. (August 2004) Nitric oxide is a regulator of hematopoietic stem cell activity. Mol Ther, 10 (2). pp. 241-8. ISSN 1525-0016 (Print)

DOI: 10.1016/j.ymthe.2004.05.030


Hematopoietic stem cells give rise to various multipotent progenitor populations, which expand in response to cytokines and which ultimately generate all of the elements of the blood. Here we show that it is possible to increase the number of stem and progenitor cells in the bone marrow (BM) by suppressing the activity of NO synthases (NOS). Exposure of mice to NOS inhibitors, either directly or after irradiation and BM transplantation, increases the number of stem cells in the BM. In the transplantation model, this increase is followed by a transient increase in the number of neutrophils in the peripheral blood. Thus, our results indicate that NO is important for the control of hematopoietic stem cells in the BM. They further suggest that suppression of NO synthase activity may allow expansion of the number of hematopoietic stem and progenitor cells or neutrophils for therapeutic purposes.

Item Type: Paper
Uncontrolled Keywords: Animals Bone Marrow drug effects radiation effects Bone Marrow Transplantation Cell Proliferation Enzyme Inhibitors pharmacology Female Gamma Rays Gene Expression Hematopoietic Stem Cells enzymology physiology Male Mice NG-Nitroarginine Methyl Ester pharmacology Neutrophils physiology Nitric Oxide physiology Nitric Oxide Synthase antagonists & inhibitors genetics metabolism RNA, Messenger analysis metabolism
Subjects: bioinformatics > genomics and proteomics > small molecules > nitric oxide
therapies > stem cells
CSHL Authors:
Communities: CSHL labs > Enikopolov lab
Depositing User: CSHL Librarian
Date: August 2004
Date Deposited: 31 Jan 2012 15:41
Last Modified: 01 Mar 2017 21:24
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